Team:TU Delft/Project/rbs-characterization/parts

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CharacterizationResultsParts

BioBricks, the making of the RBS Characterization

BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations.

Transformation into competent Top10 E.coli cells yielded positives, as determined by fluorescence analysis.

The cells were cultured over 18 hours in 96-well plates using a Gen5 fluorescence and absorbance plate reader, LB with ampicillin was the culture medium used in these experiments. Only the results obtained during exponential growth phase were taken into account.

Parts

Our constructs used for measurements:


J23100

J61100
GFP
E0040

B0015

J23100

J61101
GFP
E0040

B0015

J23100

J61107
GFP
E0040

B0015

J23100

J61117
GFP
E0040

B0015

J23100

J61127
GFP
E0040

B0015