Team:TU Delft/Parts/characterization

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Characterization

Our favorite parts were also the ones best characterized. This page is a quick overview of the results. You can find a complete overview of characterization on the project pages.

Favorite Parts

BBa_K398029 - ALDH

TUDelftALDH final.jpg

Our results suggest that the recombinant strain E. coli 029A functionally express our biobrick BBa_K398029. From the statistical analysis that we performed we concluded that the expression of the biobrick BBa_K398006 under the promoter-rbs combination BBa_J23100-BBa_J61117 increases the dodecanal dehydrogenase activity in E. coli cell extracts 2-fold. Moreover, the enzymatic activities measured for the construct BBa_K398029 were equivalent to 33.98% of the Pseudomonas putida aldehyde dehydrogenase activity. Read more about it in our results page

View BBa_K398029 in the parts registry

Specified Components


K398029

Designer: Kira Schipper

Status: Available

BBa_K398331 - pCaif

According to our results, the smallest part that we designed it's working!!!

pCaiF a natural promoter in E. coli that is active at high cAMP levels was characterized attaching E00240 in front of our part. Different GFP production rates were found at the stationary phase showing that GFP production depends on the amount of glucose in the medium. Moreover, growth on a secondary carbon source decreases the GFP production rate.

TU Delft pCaiF final.jpg

Read more about our fascinating results in our website!

View BBa_K398331 in the parts registry

Specified Components

pCaiF
K398326
GFP report
E0240

Designer: Kira Schipper

Status: Available

BBa_K398406 - Solvent Tolerance

The solvent tolerance cluster (BBa_K398406) was expressed on in E. coli K12. The growth of the cells was tested under different amounts of n-hexane. The results show that this part gives to the cells an growth advantage at higher n-hexane concentrations. The parental strain E. coli K12 grows really slow at 10% (v/v) of n-hexane/M9 mixture.

TU Delft Solvent tolerance.jpg

Read more about these results in our website!

View BBa_K398406 in the parts registry

Specified Components

406
K398406

Designer: Hugo Federico Cueto Rojas and Ramon van der Valk

Status: Planning

BBa_K398206 - Emulsifier

Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the BBa_K398206 characteristics
This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our solubility subproject.

View BBa_K398206 in the parts registry

Specified Components

lacI+pL
R0011

B0032

K398200

B0015

Designer: Eva Brinkman

Status: Available