Team:TU Delft/Modeling/MFA/additional pathways

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== Pathways added to the ''E. coli'' metabolic network ==
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__NOTOC__
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{{Team:TU_Delft/frame_check}}
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==Pathways added to the ''E. coli'' metabolic network==
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Tne ''E. coli'' network from Cell Net Analyzer contains, glycolysis, TCA cycle, pentose phosphate pathway, gluconeogenesis, anapleorotic routes, oxydative phosphorilization and biosynthesis pathways.
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[[Image:Team_TUDelft_NADHdehydro.png|thumb|270px|right|'''Figure 1''' – Reaction taken from [http://biocyc.org/ECOLI/NEW-IMAGE?type=REACTION-IN-PATHWAY&object=NADH-DEHYDROG-A-RXN Ecocyc]]]
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There was one change made in the standard network for the NADH dehydrogenase reaction, annotated as NADHdehydro in CellNetAnalyzer. In the network of CellNetAnalyzer this reaction exports two protons, but this was changed to 4.
+
The ''E. coli'' network from CellNetAnalyzer contains, glycolysis, TCA cycle, pentose phosphate pathway, gluconeogenesis, anapleorotic routes, oxydative phosphorilization and biosynthesis pathways. CellNetAnalyzer can be found [http://www.mpi-magdeburg.mpg.de/projects/cna/cna.html here] with this network.
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reference (in new window); http://biocyc.org/ECOLI/NEW-IMAGE?type=REACTION-IN-PATHWAY&object=NADH-DEHYDROG-A-RXN
 
 +
There was one change made in the network provided by CellNetAnalyzer for the NADH dehydrogenase reaction, annotated as NADHdehydro in CellNetAnalyzer. In the network of CellNetAnalyzer this reaction exports two protons, but this was changed to 4 as given by Ecocyc. This reaction is shown in figure 1.
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*make a reference for the pathways from CNA
+
==Alkane degradation==
-
== Alkane degradation ==
 
-
To link alkanes to the existing network, the beta-oxidation was chosen as entry point. Several genes were used to transform alkanes in to alkanoic acid which enters the beta oxydation cycle. These genes were;
+
To link alkanes to the existing network, the beta-oxidation was chosen as entry point. The reactions from several genes from biobricks were used to transform alkanes in to fatty acids which enter the beta oxidation cycle. These genes are listed below. The reactions used for CNA already leave out some redundant information. For example only NADH is used instead of NADH and NAD<sup>+</sup>.
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AlkB2 (EC 1.14.15.3) & ladA (EC )
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'''AlkB2 (EC 1.14.15.3)'''
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n-alkane + reduced rubredoxin + O2 + 2 H+ -> n-alkanol + oxidized rubredoxin + H2O
+
[[Image:Team_TUDelft_AlkB.png|thumb|270px|right|'''Figure 2''' – Reaction taken from [http://metacyc.org/META/new-image?type=REACTION&object=ALKANE-1-MONOOXYGENASE-RXN Metacyc]]]
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http://metacyc.org/META/new-image?type=REACTION&object=ALKANE-1-MONOOXYGENASE-RXN
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The reaction for AlkB2 is shown in figure 2.
 +
The reaction was implemented in CNA as;
-
RubA3/RubA4 (EC 1.18.1.1)
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n-alkane + reduced rubredoxin + O2 -> n-alkanol + oxidized rubredoxin
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Oxidized rubredoxin + NADH -> reduced rubredoxin + NAD+ + H+
 
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http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RUBREDOXIN--NAD%2b-REDUCTASE-RXN
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'''RubA3/RubA4 (EC 1.18.1.1)'''
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ADH (EC 1.1.1.1)
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[[Image:Team_TUDelft_EC1672.png|thumb|270px|right|'''Figure 3''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RUBREDOXIN--NAD%2b-REDUCTASE-RXN Metacyc]]]
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n-alkanol + NAD+ -> n-aldehyde + NADH
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The reaction for the regeneration of rubredoxin is shown in figure 3.
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http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=ALCOHOL-DEHYDROG-GENERIC-RXN
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The reaction was implemented in CNA as;
 +
oxidized rubredoxin + NADH -> reduced rubredoxin
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ALDH (EC 1.2.1.3)
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This and the previous reaction are performed by this [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398014 BioBrick] and this [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398017 BioBrick]
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n-aldehyde + NAD+ + CoA -> n-fatty acid acid + NADH
 
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http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-4142
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'''ADH (EC 1.1.1.1)'''
 +
[[Image:Team_TUDelft_ADH.png|thumb|270px|right|'''Figure 4''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=ALCOHOL-DEHYDROG-GENERIC-RXN Metacyc]]]
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From here the genes are already present in ''E. coli''
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The reaction for ADH is shown in figure 4.
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fatty acyl-CoA synthetase (EC 6.2.1.3)
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The reaction was implemented in CNA as;
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n-fatty acid + ATP + CoA -> n-saturated fatty acyl-CoA + AMP
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n-alkanol -> n-aldehyde + NADH
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http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=ACYLCOASYN-RXN
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This reaction is performed by this [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398018 BioBrick]
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Adenylate kinase (EC 2.7.4.3)
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'''ALDH (EC 1.2.1.3)'''
 +
 
 +
[[Image:Team_TUDelft_ALDH.png|thumb|270px|right|'''Figure 5''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-4142 Metacyc]]]
 +
 
 +
The reaction for ALDH is shown in figure 5.
 +
 
 +
The reaction was implemented in CNA as;
 +
 
 +
n-aldehyde -> n-fatty acid + NADH
 +
 
 +
This reaction is performed by this [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398029 BioBrick]
 +
 
 +
 
 +
'''fatty acyl-CoA synthetase (EC 6.2.1.3)'''
 +
 
 +
[[Image:Team_TUDelft_fadK.png|thumb|270px|right|'''Figure 6''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=ACYLCOASYN-RXN Ecocyc]]]
 +
 
 +
From here the genes are already present in the ''E. coli'' genome. They were not yet present in the metabolic network for ''E. coli'' in CellNetAnalyzer however. So the following reactions and pathways were also implemented in CNA. The reaction for fatty acyl-CoA synthetase is shown in figure 6.
 +
 
 +
The reaction was implemented in CNA as;
 +
 
 +
n-fatty acid + ATP -> n-saturated fatty acyl-CoA + AMP
 +
 
 +
 
 +
'''Adenylate kinase (EC 2.7.4.3)'''
 +
 
 +
[[Image:Team_TUDelft_adk.png|thumb|270px|right|'''Figure 7''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=ADENYL-KIN-RXN Ecocyc]]]
 +
 
 +
CNA did not have a reaction to regenerate AMP yet, so the reaction for adenylate kinase was added to the network. The reaction for adenylate kinase is shown in figure 7.
 +
 
 +
The reaction was implemented in CNA as;
ATP + AMP -> 2 ADP
ATP + AMP -> 2 ADP
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http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=ADENYL-KIN-RXN
 
 +
'''Fatty acid beta-oxidation cycle (EC 1.3.99.3    EC 4.2.1.17    EC 1.1.1.35    EC 2.3.1.16)'''
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Fatty acid beta-oxidation cycle (EC 1.3.99.3    EC 4.2.1.17    EC 1.1.1.35    EC 2.3.1.16)
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[[Image:Team_TUDelft_fa_oxy.png|thumb|270px|right|'''Figure 8''' – Reaction taken from [http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=FAO-PWY&detail-level=2 Ecocyc]]]
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n-saturated fatty acyl-CoA + FAD + NAD+ + CoA ↔ (n - 2)-saturated fatty acyl-CoA + acetyl-CoA + FADH2 + NADH
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The reactions for this pathway are shown in figure 8.
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http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=FAO-PWY&detail-level=2
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The lumped reaction was implemented in CNA as;
-
== Biomass formation ==
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n-saturated fatty acyl-CoA -> (n - 2)-saturated fatty acyl-CoA + acetyl-CoA + FADH<sub>2</sub> + NADH
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The biomass is formed by many anabolic reactions that make monomers. All the anabolic reactions start at the so called key metabolites. There are 12 key metabolites and they are all in the glycolytic pathway and the TCA cycle. In the tool they are the red metabolites.
 
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== NO3 as electron acceptor ==
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'''FADH<sub>2</sub> regeneration'''
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In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.
+
The cofactor FADH<sub>2</sub> is not yet defined in the network of CNA, so a reaction had to be introduced to regenerate it. FADH<sub>2</sub> gives its electrons to ubiquinol just like NADH, however in this process no protons are exported.
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The standard oxydative phosphorylation is showed here; (EC 1.6.5.3  EC 1.10.2.-)
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The reaction was implemented in CNA as;
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http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=PWY0-1335
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1 FADH<sub>2</sub> -> 1 QH<sub>2</sub>
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The second step will be disabled in the network and be replaced with a nitrate reductase (EC 1.7.99.4);
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'''Odd numbered alkanes'''
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*note: make link open up a new window
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For all even numbered alkanes the above reactions completely link them to the main network in CNA. All the co-factors have regeneration reactions and all the alkanes are converted into acetyl-CoA. The final reaction in the beta oxidation cycle (n = 4) produces then two acetyl-CoA. For odd numbered alkanes the final reaction however (n = 5), a propionyl-CoA is generated;
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http://biocyc.org/ECOLI/NEW-IMAGE?type=REACTION&object=RXN0-6369
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n-saturated fatty acyl-CoA -> propionyl-CoA + acetyl-CoA + FADH<sub>2</sub> + NADH
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The reaction for CNA will be;
 
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NO3- + QH2 -> NO2-
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'''2-methylcitrate cycle (EC 2.3.3.5    EC 4.2.1.79    EC 4.2.1.99    EC 4.1.3.30)'''
 +
[[Image:Team_TUDelft_propionylCoA.png|thumb|270px|right|'''Figure 9''' – Reaction taken from [http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=PWY0-42&detail-level=2 Ecocyc]]]
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This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP. Less protons are exported per mol of NADH, so the ATP/NADH ratio will drop compared to oxygen. The goal of implementing this pathway however, is to see how much the oxygen requirement of ''E. coli'' can be reduced.
+
This propionyl-CoA still needs to be linked to the main network in CNA. The pathway that was used to process this metabolite, was a part of the 2-methylcitrate cycle. The reactions for this pathway are shown in figure 9.
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== PHB production ==
+
The lumped reaction was implemented in CNA as;
-
In previous situations the hydrocarbons were degraded only to form biomass and CO2. It is interesting to see how much product could be made from hydrocarbons. PHB is a polymer of polyhydroxybutyrate. The production pathway of PHB is well known. PHB is a solid product which is to recover in the down stream process.
+
propionyl-CoA + oxaloacetic acid -> succinate + pyruvate
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*note: make link open up a new window
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==Biomass formation==
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The pathway is displayed here (EC 2.3.19  EC 1.1.1.36  EC 2.3.1.-)
+
-
http://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY1-3&detail-level=2
 
 +
The biomass is formed by many anabolic reactions that make monomers. All the anabolic reactions start at the so called key metabolites. There are 12 key metabolites and they are all in the glycolytic pathway and the TCA cycle. In the tool they are the red metabolites. For this analysis the anabolic reactions already present in CNA were used. These are a lot of reactions however and they will not be shown in this analysis, although they do occur. In the tool growth can ben seen as consumption of the key metabolites.
-
In this scenario the lumped PHB production pathway was added to metabolic network;
+
==NO3 as electron acceptor==
 +
 
 +
 
 +
[[Image:Team_TUDelft_NADH_regen.png|thumb|270px|right|'''Figure 10''' – Reaction taken from [http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=PWY0-1335 Ecocyc]]]
 +
 
 +
In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.
 +
 
 +
The standard oxidative phosphorylation (EC 1.6.5.3  EC 1.10.2.-) is shown in figure 10.
 +
 
 +
[[Image:Team_TUDelft_NOx.png|thumb|270px|right|'''Figure 11''' – Reaction taken from [http://biocyc.org/ECOLI/NEW-IMAGE?type=REACTION&object=RXN0-6369 Ecocyc]]]
 +
 
 +
The second step will be disabled in the network and be replaced with a nitrate reductase (EC 1.7.99.4). This reaction is shown in figure 11.
 +
 
 +
The reaction was implemented in CNA as;
 +
 
 +
NO<sub>3</sub><sup>-</sup> + QH<sub>2</sub> -> NO<sub>2</sub><sup>-</sup>
 +
 
 +
This reaction uses NO<sub>3</sub> as an electron acceptor to regenerate NADH and export protons to generate ATP. Less protons are exported per mol of NADH in comparison with oxygen as electron acceptor, so the ATP/NADH ratio will drop. The goal of implementing this pathway however, is to see how much the oxygen requirement of ''E. coli'' can maximally be reduced.
 +
 
 +
==PHB production==
 +
 
 +
[[Image:Team_TUDelft_PHB.png|thumb|270px|right|'''Figure 12''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY1-3&detail-level=2 Metacyc]]]
 +
 
 +
In previous situations the hydrocarbons were degraded only to form biomass and CO<sub>2</sub>. It is interesting to see how much product theoretically could be made from hydrocarbons. PHB is a polymer of polyhydroxybutyrate. The production pathway of PHB is well known. PHB is a solid product which is easy to recover in the down stream process.
 +
 
 +
The pathway is displayed in figure 12. The enzymes present in this pathway are EC 2.3.19, EC 1.1.1.36 and EC 2.3.1.-.
 +
 
 +
The lumped reaction was implemented in CNA as;
2 acetyl-CoA + NADPH -> (R)-3-hydroxybutanoyl-CoA
2 acetyl-CoA + NADPH -> (R)-3-hydroxybutanoyl-CoA
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the polymerization reaction just consumes (R)-3-hydroxybutanoyl-CoA.
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the polymerization reaction just consumes (R)-3-hydroxybutanoyl-CoA, because a solid has no concentration in the liquid and therefore does not need to fulfill the steady-state condition. There are limiting factors to this reaction, but those are not considered in this analysis.
 +
 
 +
==Hydrogen production==
 +
 
 +
[[Image:Team_TUDelft_H2.png|thumb|270px|right|'''Figure 13''' – Reaction taken from [http://biocyc.org/ECOLI/NEW-IMAGE?type=REACTION&object=FHLMULTI-RXN Ecocyc]]]
 +
 
 +
As with PHB, this pathway was added to the metabolic pathway to see how much product could be made from the alkane degradation in stead of just biomass and CO<sub>2</sub> formation. Hydrogen is considered a green fuel as its combustion produces water. Hydrogen is a volatile product and is also easily separated from fermentation broth. It does however contain no carbon atoms, so hydrogen production will still result in production of CO<sub>2</sub> and biomass.
 +
 
 +
The pathway is shown in figure 13
 +
 
 +
The reaction was implemented in CNA as;
 +
 
 +
Formate -> H<sub>2</sub> + CO<sub>2</sub>
 +
 
 +
==Isoprene production==
 +
 
 +
[[Image:Team_TUDelft_isoprene.png|thumb|270px|right|'''Figure 14''' – Reaction taken from [http://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-6270&detail-level=2 Metacyc]]]
 +
 
 +
As with the previous product pathways, isoprene is a product that can easily be separated from liquid. Isoprene is a volatile substance found in plants. ''E. coli'' will not be able to produce is in the near future, but it is an interesting product. It is a very reduced product, with a similar amount of electron per carbon atom as alkanes. Alkanes have 6 - 6.3 electrons per carbon atom depending on the length and isoprene has 5.6 electron per carbon electron.
 +
 
 +
The pathway is displayed in figure 14. The enzymes in the pathway are EC 2.2.1.7, EC 1.1.1.267, EC 2.7.7.60, EC 2.7.1.148, EC 4.6.1.12, EC 1.17.7.1, EC 1.17.1.2 and EC 4.2.3.27. The reaction of enzyme EC 1.17.7.1 has been adjusted to generate only 1 oxidized ferredoxin. This was done to hold the electron balance. Ferredoxin is regenerated by consuming a NADH.
 +
 
 +
The lumped reaction was implemented in CNA as;
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 +
1 pyruvate + 1 D-glyceraldehyde-3-phosphate + 1 NADPH + 2 NADH + 3 ATP -> isoprene + CO2
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 +
 
 +
 
 +
Go to the detailed results of the metabolic flux analysis [[Team:TU_Delft/Modeling/MFA/results|here]]
 +
 
 +
Go back the metabolic flux analysis main page [[Team:TU_Delft/Modeling/MFA|here]]
 +
 
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== isoprene production ==
 
-
In previous situations the hydrocarbons were degraded only to form biomass and CO2. It is interesting to see how much product could be made from hydrocarbons. Isoprene is a volatile product found in plants. ''E. coli'' will not be able to produce is in the near future, but it is an interesting product It is a very reduced product, with a similar amount of electron per carbon atom. Hydrocarbons have 6 - 6.3 electrons per carbon atom depending on the length and isoprene has 5.6 electron per carbon electron. ''If these values are close to each other, it has a positive influence on the maximal theoretical yield.'' Also the volatile nature of isoprene is very favorable for the downstream process
 
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*note: make link open up a new window
 
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The pathway is displayed here http://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-6270&detail-level=2
 
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In this scenario the lumped isoprene production pathway was added to metabolic network;
 
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1 pyruvate + 1 D-glyceraldehyde-3-phosphate + 1 NADPH + 3 NADH + 3 ATP -> isoprene + CO2
 
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isoprene export
 
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== hydrogen production ==
 
-
In previous situations the hydrocarbons were degraded only to form biomass and CO2. It is interesting to see how much product could be made from hydrocarbons. Hydrogen is considered a green fuel. Hydrogen is a volatile product and is easily separated from fermentation broth. It does however contain no carbon atoms, so hydrogen will result in production of CO2 and biomass.
 
-
*note: make link open up a new window
 
-
The pathway is displayed here http://biocyc.org/ECOLI/NEW-IMAGE?type=REACTION&object=FHLMULTI-RXN
 
-
In this scenario the hydrogen production pathway was added to metabolic network;
 
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Formate + H+ -> H2 + CO2
 
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hydrogen export
 
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[[Team:TU_Delft/Modeling/MFA|go back]]
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Latest revision as of 14:44, 27 October 2010

Pathways added to the E. coli metabolic network

Figure 1 – Reaction taken from Ecocyc

The E. coli network from CellNetAnalyzer contains, glycolysis, TCA cycle, pentose phosphate pathway, gluconeogenesis, anapleorotic routes, oxydative phosphorilization and biosynthesis pathways. CellNetAnalyzer can be found here with this network.


There was one change made in the network provided by CellNetAnalyzer for the NADH dehydrogenase reaction, annotated as NADHdehydro in CellNetAnalyzer. In the network of CellNetAnalyzer this reaction exports two protons, but this was changed to 4 as given by Ecocyc. This reaction is shown in figure 1.

Alkane degradation

To link alkanes to the existing network, the beta-oxidation was chosen as entry point. The reactions from several genes from biobricks were used to transform alkanes in to fatty acids which enter the beta oxidation cycle. These genes are listed below. The reactions used for CNA already leave out some redundant information. For example only NADH is used instead of NADH and NAD+.


AlkB2 (EC 1.14.15.3)

Figure 2 – Reaction taken from Metacyc

The reaction for AlkB2 is shown in figure 2.

The reaction was implemented in CNA as;

n-alkane + reduced rubredoxin + O2 -> n-alkanol + oxidized rubredoxin


RubA3/RubA4 (EC 1.18.1.1)

Figure 3 – Reaction taken from Metacyc

The reaction for the regeneration of rubredoxin is shown in figure 3.

The reaction was implemented in CNA as;

oxidized rubredoxin + NADH -> reduced rubredoxin

This and the previous reaction are performed by this BioBrick and this BioBrick


ADH (EC 1.1.1.1)

Figure 4 – Reaction taken from Metacyc

The reaction for ADH is shown in figure 4.

The reaction was implemented in CNA as;

n-alkanol -> n-aldehyde + NADH

This reaction is performed by this BioBrick


ALDH (EC 1.2.1.3)

Figure 5 – Reaction taken from Metacyc

The reaction for ALDH is shown in figure 5.

The reaction was implemented in CNA as;

n-aldehyde -> n-fatty acid + NADH

This reaction is performed by this BioBrick


fatty acyl-CoA synthetase (EC 6.2.1.3)

Figure 6 – Reaction taken from Ecocyc

From here the genes are already present in the E. coli genome. They were not yet present in the metabolic network for E. coli in CellNetAnalyzer however. So the following reactions and pathways were also implemented in CNA. The reaction for fatty acyl-CoA synthetase is shown in figure 6.

The reaction was implemented in CNA as;

n-fatty acid + ATP -> n-saturated fatty acyl-CoA + AMP


Adenylate kinase (EC 2.7.4.3)

Figure 7 – Reaction taken from Ecocyc

CNA did not have a reaction to regenerate AMP yet, so the reaction for adenylate kinase was added to the network. The reaction for adenylate kinase is shown in figure 7.

The reaction was implemented in CNA as;

ATP + AMP -> 2 ADP


Fatty acid beta-oxidation cycle (EC 1.3.99.3 EC 4.2.1.17 EC 1.1.1.35 EC 2.3.1.16)

Figure 8 – Reaction taken from Ecocyc

The reactions for this pathway are shown in figure 8.

The lumped reaction was implemented in CNA as;

n-saturated fatty acyl-CoA -> (n - 2)-saturated fatty acyl-CoA + acetyl-CoA + FADH2 + NADH


FADH2 regeneration

The cofactor FADH2 is not yet defined in the network of CNA, so a reaction had to be introduced to regenerate it. FADH2 gives its electrons to ubiquinol just like NADH, however in this process no protons are exported.

The reaction was implemented in CNA as;

1 FADH2 -> 1 QH2


Odd numbered alkanes

For all even numbered alkanes the above reactions completely link them to the main network in CNA. All the co-factors have regeneration reactions and all the alkanes are converted into acetyl-CoA. The final reaction in the beta oxidation cycle (n = 4) produces then two acetyl-CoA. For odd numbered alkanes the final reaction however (n = 5), a propionyl-CoA is generated;

n-saturated fatty acyl-CoA -> propionyl-CoA + acetyl-CoA + FADH2 + NADH


2-methylcitrate cycle (EC 2.3.3.5 EC 4.2.1.79 EC 4.2.1.99 EC 4.1.3.30)

Figure 9 – Reaction taken from Ecocyc

This propionyl-CoA still needs to be linked to the main network in CNA. The pathway that was used to process this metabolite, was a part of the 2-methylcitrate cycle. The reactions for this pathway are shown in figure 9.

The lumped reaction was implemented in CNA as;

propionyl-CoA + oxaloacetic acid -> succinate + pyruvate

Biomass formation

The biomass is formed by many anabolic reactions that make monomers. All the anabolic reactions start at the so called key metabolites. There are 12 key metabolites and they are all in the glycolytic pathway and the TCA cycle. In the tool they are the red metabolites. For this analysis the anabolic reactions already present in CNA were used. These are a lot of reactions however and they will not be shown in this analysis, although they do occur. In the tool growth can ben seen as consumption of the key metabolites.

NO3 as electron acceptor

Figure 10 – Reaction taken from Ecocyc

In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.

The standard oxidative phosphorylation (EC 1.6.5.3 EC 1.10.2.-) is shown in figure 10.

Figure 11 – Reaction taken from Ecocyc

The second step will be disabled in the network and be replaced with a nitrate reductase (EC 1.7.99.4). This reaction is shown in figure 11.

The reaction was implemented in CNA as;

NO3- + QH2 -> NO2-

This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP. Less protons are exported per mol of NADH in comparison with oxygen as electron acceptor, so the ATP/NADH ratio will drop. The goal of implementing this pathway however, is to see how much the oxygen requirement of E. coli can maximally be reduced.

PHB production

Figure 12 – Reaction taken from Metacyc

In previous situations the hydrocarbons were degraded only to form biomass and CO2. It is interesting to see how much product theoretically could be made from hydrocarbons. PHB is a polymer of polyhydroxybutyrate. The production pathway of PHB is well known. PHB is a solid product which is easy to recover in the down stream process.

The pathway is displayed in figure 12. The enzymes present in this pathway are EC 2.3.19, EC 1.1.1.36 and EC 2.3.1.-.

The lumped reaction was implemented in CNA as;

2 acetyl-CoA + NADPH -> (R)-3-hydroxybutanoyl-CoA

the polymerization reaction just consumes (R)-3-hydroxybutanoyl-CoA, because a solid has no concentration in the liquid and therefore does not need to fulfill the steady-state condition. There are limiting factors to this reaction, but those are not considered in this analysis.

Hydrogen production

Figure 13 – Reaction taken from Ecocyc

As with PHB, this pathway was added to the metabolic pathway to see how much product could be made from the alkane degradation in stead of just biomass and CO2 formation. Hydrogen is considered a green fuel as its combustion produces water. Hydrogen is a volatile product and is also easily separated from fermentation broth. It does however contain no carbon atoms, so hydrogen production will still result in production of CO2 and biomass.

The pathway is shown in figure 13

The reaction was implemented in CNA as;

Formate -> H2 + CO2

Isoprene production

Figure 14 – Reaction taken from Metacyc

As with the previous product pathways, isoprene is a product that can easily be separated from liquid. Isoprene is a volatile substance found in plants. E. coli will not be able to produce is in the near future, but it is an interesting product. It is a very reduced product, with a similar amount of electron per carbon atom as alkanes. Alkanes have 6 - 6.3 electrons per carbon atom depending on the length and isoprene has 5.6 electron per carbon electron.

The pathway is displayed in figure 14. The enzymes in the pathway are EC 2.2.1.7, EC 1.1.1.267, EC 2.7.7.60, EC 2.7.1.148, EC 4.6.1.12, EC 1.17.7.1, EC 1.17.1.2 and EC 4.2.3.27. The reaction of enzyme EC 1.17.7.1 has been adjusted to generate only 1 oxidized ferredoxin. This was done to hold the electron balance. Ferredoxin is regenerated by consuming a NADH.

The lumped reaction was implemented in CNA as;

1 pyruvate + 1 D-glyceraldehyde-3-phosphate + 1 NADPH + 2 NADH + 3 ATP -> isoprene + CO2


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