Team:TU Delft/Modeling/MFA/NO3


Revision as of 09:06, 13 September 2010 by Lbergwerff (Talk | contribs)

NO3 as electron acceptor

In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.

In this scenario the oxidative phosphorylation was disabled and a replacement was implemented;

NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 6 Hex+ + H2O NO3 import NO2 export

This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP.

The goal of implementing this pathway is to reduce the oxygen requirement of E. coli.

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