Team:TU Delft/Modeling/MFA


Revision as of 13:44, 23 October 2010 by Lbergwerff (Talk | contribs)

Metabolic Flux Analysis

A metabolic flux analysis (MFA) is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to E. coli along with the hydrocarbon degradation from our BioBricks to see what the maximal theoretical yields on alkanes are compared to glucose.

Are you not familiar with MFAs? Read more about how a metabolic flux analysis works here.

For our MFA a Matlab application, Cell Net Analyzer (CNA), was used. CNA already contains a standard simplified E. coli metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. The resulting network is the network used for adding additional pathways.

Would you like to more about the details of the implemented pathways? Read the Detailed MFA results page.

The fluxes that were calculated for maximal yields with CellNetAnalyzer were exported and inputted into Omix. Omix is a tool for drawing networks in general, but lends itself espescially well for biochemical pathways. From the networks that were drawn with Omix we made a tool in which you yourself can select different substrates and products and see what pathways are used in an ideal situation. The tool is below this text

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Visualization of pathways