# Team:TU Delft/Modeling/MFA

### From 2010.igem.org

(Difference between revisions)

Lbergwerff (Talk | contribs) (→Metabolic Flux Analysis) |
Lbergwerff (Talk | contribs) (→Metabolic Flux Analysis) |
||

Line 2: | Line 2: | ||

==Metabolic Flux Analysis== | ==Metabolic Flux Analysis== | ||

- | A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. | + | A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to ''E. coli'' along with the hydrocarbon degradation from our biobricks to see what the maximal theoretical yields on alkanes are compared to glucose. |

How a metabolic flux analysis works, is explained [[Team:TU_Delft/Modeling/MFA/explanation|here.]] | How a metabolic flux analysis works, is explained [[Team:TU_Delft/Modeling/MFA/explanation|here.]] | ||

- | For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified ''E. coli'' metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. | + | For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified ''E. coli'' metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. The resulting network is the network used for adding additional pathways. |

- | + | ||

Implemented pathways | Implemented pathways |

## Revision as of 13:57, 7 October 2010

## Metabolic Flux Analysis

A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to *E. coli* along with the hydrocarbon degradation from our biobricks to see what the maximal theoretical yields on alkanes are compared to glucose.

How a metabolic flux analysis works, is explained here.

For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified *E. coli* metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. The resulting network is the network used for adding additional pathways.

Implemented pathways

## Visualization of pathways

refer to detailed results page here