Team:TU Delft/Modeling/HC regulation/Sensitivity
From 2010.igem.org
Sensitivity analysis
For all beta parameters (lumped variables for transcriptions speed, translations speed, promoter strength and rbs strength) and all K parameters (equilibrium constant for binding of AlkS with alkanes and coefficients for the activity of promoters) a sensitivity analysis has been performed. This was done by varying the parameters from 50% of their original values to 200% of their original values.
For this range of parameter values it is checked how much the steady state concentration of AlkB is and how long it takes for 95% of the steady state concentration of AlkB to be reached from steady state values for no alkanes to steady state values for 1 µM of alkanes.
In the figures below are the results for this sensitivity analysis.
These results are summarized in table 1. A '0' means insensitive, a '+' means somewhat sensitive and a '++' is very sensitive.
Table 1; results for sensitivity analysis for the gene regulation model
Parameter | Sensitive for steady state concentration | Sensitive for reaching steady state concentration | ||||||||||||||||||||||||
β^{max}_{S1} | 0 | + | β^{max}_{S2} | + | + | β^{max}_{B} | ++ | 0 | β^{max}_{AlkS} | + | + | β^{max}_{AlkB} | ++ | 0 | K_{HC} | + | + | K_{S1} | 0 | + | K_{S2} | + | + | K_{B} | + | + |
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