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Team:TU Delft/9 May 2010 content
From 2010.igem.org
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| - | + | =Session 11= | |
First meeting after have chosen our subject. | First meeting after have chosen our subject. | ||
| - | + | ===Planning=== | |
Made a preliminary planning: | Made a preliminary planning: | ||
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* June: Lab work! | * June: Lab work! | ||
| - | + | [[Image:TU_Delft_DSC_9760.JPG|400px|thumb|left|Meeting at Aljosha's place]] | |
| + | |||
| + | ===Team Division=== | ||
As already mentioned, our subject can be divided into 4 subparts, but each subpart will need team members as well as an initial plan of action. | As already mentioned, our subject can be divided into 4 subparts, but each subpart will need team members as well as an initial plan of action. | ||
| - | + | ====1. Hydrocarbon degradation==== | |
'''Team members:''' Hugo, Mathias & Kira | '''Team members:''' Hugo, Mathias & Kira | ||
*Focus on alkenes | *Focus on alkenes | ||
| Line 21: | Line 23: | ||
* Drug efflux pump will create Drug resistance in E.coli (ethical subject!), Pseudomonas & E.coli membranes are pretty similar according to Hugo | * Drug efflux pump will create Drug resistance in E.coli (ethical subject!), Pseudomonas & E.coli membranes are pretty similar according to Hugo | ||
| - | + | ====2. Salt tolerance==== | |
| - | '''Team members:'''Nadine, Mia | + | '''Team members:''' Nadine, Mia |
* Salt in the solution: test with sodium chloride | * Salt in the solution: test with sodium chloride | ||
* Conductivity or polarity measurements? | * Conductivity or polarity measurements? | ||
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* Regulation system? Sensor? Existing biobrick? | * Regulation system? Sensor? Existing biobrick? | ||
| - | + | ====3. Emulsifier==== | |
| - | '''Team members:'''Pieter, Eva | + | '''Team members:''' Pieter, Eva |
*First look at hydrocarbon degrading organisms first, and what kind of emulsifier they excrete and see if we can use this (see nature review on huddle for further reference) | *First look at hydrocarbon degrading organisms first, and what kind of emulsifier they excrete and see if we can use this (see nature review on huddle for further reference) | ||
* Designing our own peptide? (but this would be a bit more difficult, but possible, no more than 16 aa!) | * Designing our own peptide? (but this would be a bit more difficult, but possible, no more than 16 aa!) | ||
| Line 37: | Line 39: | ||
* Start by choosing the emulsifier | * Start by choosing the emulsifier | ||
| - | + | ====4. Modeling & possible products==== | |
| - | '''Team members:'''Jelmer, Luke | + | '''Team members:''' Jelmer, Luke |
* MFA (3 days?) Alkene degradation: look at SB assignment… | * MFA (3 days?) Alkene degradation: look at SB assignment… | ||
* Thermodynamics? | * Thermodynamics? | ||
| Line 47: | Line 49: | ||
* Regulation strategy for salt tolerance? | * Regulation strategy for salt tolerance? | ||
* PHB, butanol or ethanol? | * PHB, butanol or ethanol? | ||
| + | |||
| + | |||
| + | =iJAM session 1= | ||
| + | Our first iJAM session! | ||
| + | The pictures were added to the iJAM album on [http://www.facebook.com/album.php?aid=22234&id=112242102125963 Facebook] | ||
Latest revision as of 19:09, 2 August 2010
Contents |
Session 11
First meeting after have chosen our subject.
Planning
Made a preliminary planning:
- Today, team division and general discussion on how to approach things
- Next meeting: Preliminary plan on general approach per group, possible questions for the whole group, and a general discussion. When making the lab-plans, also incorporate deadlines at which goals must be reached.
- Following, weekly meetings to keep everyone up to date?
- End of May: Meeting with the professors / staff of kluyver etc in which each group’s plan will be presented, and we will ask the people attending to give advise / comments / remarks
- June: Lab work!
Team Division
As already mentioned, our subject can be divided into 4 subparts, but each subpart will need team members as well as an initial plan of action.
1. Hydrocarbon degradation
Team members: Hugo, Mathias & Kira
- Focus on alkenes
- Paper by Rojo: 3 main enzymes, Make different strains for each type of alkene degradation (different enzymes for different lengths), Gene’s are in a cluster, so design a primer for this cluster,
- Using GC for measuring the alkenes?
- Hydrocarbon Resistance: Paper by Lazaroaie => This is phase 2! First start with a “normal” strain under mild conditions – check how high the normal resistance is?
- Drug efflux pump will create Drug resistance in E.coli (ethical subject!), Pseudomonas & E.coli membranes are pretty similar according to Hugo
2. Salt tolerance
Team members: Nadine, Mia
- Salt in the solution: test with sodium chloride
- Conductivity or polarity measurements?
- Paper with salt tolerancein E.coli: Kapardra
- Biobrick: not well characterized? Check presentation? If not: Characterize it?
- Ecotin? (osmoprotectant)
- Regulation system? Sensor? Existing biobrick?
3. Emulsifier
Team members: Pieter, Eva
- First look at hydrocarbon degrading organisms first, and what kind of emulsifier they excrete and see if we can use this (see nature review on huddle for further reference)
- Designing our own peptide? (but this would be a bit more difficult, but possible, no more than 16 aa!)
- Export would be the main problem? (Self-destroying population?)
- Start by choosing the emulsifier
4. Modeling & possible products
Team members: Jelmer, Luke
- MFA (3 days?) Alkene degradation: look at SB assignment…
- Thermodynamics?
- Prediction of growthrate (Heijnen hypothesis?)
- Prediction of emulsion capacity
- Molecular dynamics simulation
- Towards the end of the project model if we could put everything together?
- Regulation strategy for salt tolerance?
- PHB, butanol or ethanol?
iJAM session 1
Our first iJAM session! The pictures were added to the iJAM album on [http://www.facebook.com/album.php?aid=22234&id=112242102125963 Facebook]
