Team:TU Delft/7 July 2010 content

From 2010.igem.org

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(Lab work)
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<h4>Characterization of Anderson RBS sequences</h4>  
<h4>Characterization of Anderson RBS sequences</h4>  
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[[Team:TU_Delft/protocols/transformation|Transformation]] of the ligation mix from [http://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday]
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[[Team:TU_Delft/protocols/transformation|Transformation]] of [http://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday's] ligation product into competent TOP10 cells and over night incubation.

Revision as of 13:32, 20 July 2010

Lab work

Ordered DNA stocks

All the colonies of the transformations of yesterday grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.

Characterization of Anderson RBS sequences

Transformation of yesterday's ligation product into competent TOP10 cells and over night incubation.