Team:TU Delft/7 July 2010 content

From 2010.igem.org

(Difference between revisions)
(Ordered DNA stocks)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
-
==Lab work==
+
=Lab work=
-
<h4>Ordered DNA stocks</h4>
+
==Ordered DNA stocks==
-
All the colonies of the transformations of [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday] grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.
+
All the colonies of the transformations of [https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=6_July_2010 yesterday] grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.
-
<h4>Characterization of Anderson RBS sequences</h4>
+
==Characterization of Anderson RBS sequences==
-
[[Team:TU_Delft/protocols/transformation|Transformation]] of the ligation mix from [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday]
+
[[Team:TU_Delft/protocols/transformation|Transformation]] of [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday's] ligation product into competent TOP10 cells and over night incubation.

Latest revision as of 12:36, 3 August 2010

Lab work

Ordered DNA stocks

All the colonies of the transformations of yesterday grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.

Characterization of Anderson RBS sequences

Transformation of yesterday's ligation product into competent TOP10 cells and over night incubation.