Team:TU Delft/7 July 2010 content

From 2010.igem.org

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(Lab work)
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==Lab work==
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=Lab work=
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<h4>Ordered DNA stocks</h4>
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==Ordered DNA stocks==
All the colonies of the transformations of [http://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday] grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.
All the colonies of the transformations of [http://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday] grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.
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<h4>Characterization of Anderson RBS sequences</h4>
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==Characterization of Anderson RBS sequences==
[[Team:TU_Delft/protocols/transformation|Transformation]] of [http://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday's] ligation product into competent TOP10 cells and over night incubation.
[[Team:TU_Delft/protocols/transformation|Transformation]] of [http://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 yesterday's] ligation product into competent TOP10 cells and over night incubation.

Revision as of 19:23, 2 August 2010

Lab work

Ordered DNA stocks

All the colonies of the transformations of yesterday grew. At the end of the day we picked colonies and grew them overnight in 200 mL LB medium containing the appropriate antibiotic.

Characterization of Anderson RBS sequences

Transformation of yesterday's ligation product into competent TOP10 cells and over night incubation.