Team:TU Delft/6 July 2010 content

From 2010.igem.org

(Difference between revisions)
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1. AlkB2 on 100 μg/mL Ampicillin
1. AlkB2 on 100 μg/mL Ampicillin
 +
2. rubA3 on 100 μg/mL Ampicillin
2. rubA3 on 100 μg/mL Ampicillin
 +
3. rubA4 on 100 μg/mL Ampicillin
3. rubA4 on 100 μg/mL Ampicillin
 +
4. rubB on 50 μg/mL Kanamycin
4. rubB on 50 μg/mL Kanamycin
 +
5. ladA on 100 μg/mL Ampicillin
5. ladA on 100 μg/mL Ampicillin
 +
6. ADH on 100 μg/mL Ampicillin
6. ADH on 100 μg/mL Ampicillin
 +
7. ALDH on 50 μg/mL Kanamycin
7. ALDH on 50 μg/mL Kanamycin
 +
8. bbc1 on 100 μg/mL Ampicillin
8. bbc1 on 100 μg/mL Ampicillin
 +
9. AlnA on 100 μg/mL Ampicillin
9. AlnA on 100 μg/mL Ampicillin
 +
10. OprG on 100 μg/mL Ampicillin
10. OprG on 100 μg/mL Ampicillin
 +
11. PalkS1-2 on 100 μg/mL Ampicillin
11. PalkS1-2 on 100 μg/mL Ampicillin
 +
12. PalkB on 100 μg/mL Ampicillin
12. PalkB on 100 μg/mL Ampicillin
 +
13. P(CaiF) on 100 μg/mL Ampenicillin
13. P(CaiF) on 100 μg/mL Ampenicillin
 +
14. AlkS on 50 μg/mL Kanamycin
14. AlkS on 50 μg/mL Kanamycin
 +
15. PhPFD-α on 100 μg/mL Ampicillin
15. PhPFD-α on 100 μg/mL Ampicillin
 +
16. PhPFD-β on 100 μg/mL Ampicillin
16. PhPFD-β on 100 μg/mL Ampicillin

Revision as of 19:09, 12 July 2010

Lab work

The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks!

We were so excited; we immediately solved the DNA in water and performed a transformation with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic.

1. AlkB2 on 100 μg/mL Ampicillin

2. rubA3 on 100 μg/mL Ampicillin

3. rubA4 on 100 μg/mL Ampicillin

4. rubB on 50 μg/mL Kanamycin

5. ladA on 100 μg/mL Ampicillin

6. ADH on 100 μg/mL Ampicillin

7. ALDH on 50 μg/mL Kanamycin

8. bbc1 on 100 μg/mL Ampicillin

9. AlnA on 100 μg/mL Ampicillin

10. OprG on 100 μg/mL Ampicillin

11. PalkS1-2 on 100 μg/mL Ampicillin

12. PalkB on 100 μg/mL Ampicillin

13. P(CaiF) on 100 μg/mL Ampenicillin

14. AlkS on 50 μg/mL Kanamycin

15. PhPFD-α on 100 μg/mL Ampicillin

16. PhPFD-β on 100 μg/mL Ampicillin


Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples:

Hexane (mL) Tris Buffer pH 8 (mL) 10% SDS (mL)
B 1 1.1 0
100 1 1 0.1
50 1 1.05 0.05
25 1 1.075 0.025
10 1 1.09 0.01
5 1 1.095 0.005


Kampioenen!!!!

Today the Dutch team will play the semi-final of the World Cup! Hear us cheer! Stop sound

Pieter by the BBQ
We are ready for the soccer game
the Netherlands won the semi-final!