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Team:TU Delft/2 July 2010 content
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| - | + | =Lab work= | |
| - | + | ==BioBrick stocks== | |
Today we performed [[Team:TU_Delft/protocols/colony_PCR|Colony PCR]] with the transformants of the BioBricks to check if everything is what is says it is. | Today we performed [[Team:TU_Delft/protocols/colony_PCR|Colony PCR]] with the transformants of the BioBricks to check if everything is what is says it is. | ||
Results of the colony PCR on [[Team:TU_Delft/protocols/agarose_gel|1% agarose gel]] | Results of the colony PCR on [[Team:TU_Delft/protocols/agarose_gel|1% agarose gel]] | ||
[[Image:Team--TU_Delft_Colony_PCR_july_2.png|400px|thumb|left|1 % agarose of colony PCR. Gel runned 1 hour at 100 V. Of all samples 10 μL + 2 μL loadingbuffer was loaded and 5 μL was loaded of the marker]] | [[Image:Team--TU_Delft_Colony_PCR_july_2.png|400px|thumb|left|1 % agarose of colony PCR. Gel runned 1 hour at 100 V. Of all samples 10 μL + 2 μL loadingbuffer was loaded and 5 μL was loaded of the marker]] | ||
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| - | + | ==Competent cells== | |
At the same time Eva is making more [[Team:TU_Delft/protocols/colony_PCR|competent cells]]. We go through our competent cells so quickly. | At the same time Eva is making more [[Team:TU_Delft/protocols/colony_PCR|competent cells]]. We go through our competent cells so quickly. | ||
To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature. | To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature. | ||
| - | + | =World Championship= | |
We watched the World Cup game (the Netherlands - Brazil) in our iGEM room. Care was taken for many tasty snacks. Cake, chips and drinks Mmmmm. | We watched the World Cup game (the Netherlands - Brazil) in our iGEM room. Care was taken for many tasty snacks. Cake, chips and drinks Mmmmm. | ||
Latest revision as of 19:21, 2 August 2010
Contents |
Lab work
BioBrick stocks
Today we performed Colony PCR with the transformants of the BioBricks to check if everything is what is says it is.
Results of the colony PCR on 1% agarose gel
Lane description:
| # | Description | Expected lenght (bp) | Primers | Status |
| M1 | EZ load percision | n/a | n/a | n/a |
| 1 | J61100 | 293 | G00100 + G00101 | ✓ |
| 2 | J61101 | 293 | G00100 + G00101 | ✓ |
| 3 | J61107 | 293 | G00100 + G00101 | ✓ |
| 4 | J61117 | 293 | G00100 + G00101 | ✓ |
| 5 | J61127 | 293 | G00100 + G00101 | ✓ |
| 6 | B0034 | 250 | G00100 + G00101 | ✓ |
| 7 | J23109 | G00100 + G00101 | ✓ | |
| 8 | B0015 | 445 | G00100 + G00101 | ✓ |
| 9 | E0422 | 1155 | G00100 + G00101 | ✓ |
| 10 | E0240 | 1114 | G00100 + G00101 | ✓ |
| M2 | Smartladder | n/a | n/a | n/a |
Competent cells
At the same time Eva is making more competent cells. We go through our competent cells so quickly. To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature.
World Championship
We watched the World Cup game (the Netherlands - Brazil) in our iGEM room. Care was taken for many tasty snacks. Cake, chips and drinks Mmmmm.
