Team:TU Delft/2 July 2010 content
From 2010.igem.org
(Difference between revisions)
Line 46: | Line 46: | ||
|293 | |293 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 53: | Line 53: | ||
|293 | |293 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 60: | Line 60: | ||
|293 | |293 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 67: | Line 67: | ||
|293 | |293 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 74: | Line 74: | ||
|293 | |293 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 81: | Line 81: | ||
|250 | |250 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 88: | Line 88: | ||
| | | | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 95: | Line 95: | ||
|445 | |445 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 102: | Line 102: | ||
|1155 | |1155 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- | ||
Line 109: | Line 109: | ||
|1114 | |1114 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
- | | | + | |<font color=limegreen>✔</font> |
| | | | ||
|- | |- |
Revision as of 17:18, 31 July 2010
Contents |
Lab work
BioBrick stocks
Today we performed Colony PCR with the transformants of the BioBricks to check if everything is what is says it is.
Results of the colony PCR on 1% agarose gel
Lane description:
# | Description | Expected lenght (bp) | Primers | Status | Remarks |
M1 | EZ load percision | n/a | n/a | n/a | |
1 | J61100 | 293 | G00100 + G00101 | ✔ | |
2 | J61101 | 293 | G00100 + G00101 | ✔ | |
3 | J61107 | 293 | G00100 + G00101 | ✔ | |
4 | J61117 | 293 | G00100 + G00101 | ✔ | |
5 | J61127 | 293 | G00100 + G00101 | ✔ | |
6 | B0034 | 250 | G00100 + G00101 | ✔ | |
7 | J23109 | G00100 + G00101 | ✔ | ||
8 | B0015 | 445 | G00100 + G00101 | ✔ | |
9 | E0422 | 1155 | G00100 + G00101 | ✔ | |
10 | E0240 | 1114 | G00100 + G00101 | ✔ | |
M2 | Smartladder | n/a | n/a | n/a |
Competent cells
At the same time Eva is making more competent cells. We go through our competent cells so quickly. To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature.
World Championship
We watched the World Cup game (the Netherlands - Brazil) in our iGEM room. Care was taken for many tasty snacks. Cake, chips and drinks Mmmmm.