Team:TU Delft/2 July 2010 content

From 2010.igem.org

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Revision as of 20:04, 18 July 2010

Contents

Lab work

BioBrick stocks

Today we performed Colony PCR with the transformants of the BioBricks to check if everything is what is says it is.

Results of the colony PCR on 1% agarose gel

Team--TU Delft Colony PCR july 2.png











Lane description:

# Description Expected band (bp)
M1 EZ load percision (5 μL)
1 J61100 (10 μL + 2 μL loadingbuffer)
2 J61101 (10 μL + 2 μL loadingbuffer)
3 J61107 (10 μL + 2 μL loadingbuffer)
4 J61117 (10 μL + 2 μL loadingbuffer)
5 J61127 (10 μL + 2 μL loadingbuffer)
6 B0034 (10 μL + 2 μL loadingbuffer)
7 J23109 (10 μL + 2 μL loadingbuffer)
8 B0015 (10 μL + 2 μL loadingbuffer)
9 E0422 (10 μL + 2 μL loadingbuffer)
10 E0240 (10 μL + 2 μL loadingbuffer)
M2 smartladder (5 μL)

All the BioBricks have the correct size.

Competent cells

At the same time Eva is making more competent cells. We go through our competent cells so quickly. To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature.

World Championship

We watched the World Cup game (the Netherlands - Brazil) in our iGEM room. Care was taken for many tasty snacks. Cake, chips and drinks Mmmmm.

Viva Hollandia! Stop sound