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From 2010.igem.org

Lab Work

We are a bit short handed in the lab this week, because most team members have exams. Considering the fine weather outside, it is not bad to have a slow week. We still managed to pick colonies of the 15 biobricks from the distribution plates and grow them overnight in 50 mL LB medium containing Ampicillin.

Today we cultivated Pseudomonas Putida GPO1 on different substrates:

1. E2 medium without carbon source

2. E2 medium + 1% LB

3. E2 medium + 10% LB

4. E2 medium + 1% octane

5. E2 medium + 10% octane

6. LB medium + 10% cyclohexane