Team:TU Delft/29 June 2010 content

From 2010.igem.org

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===Lab Work===
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==Lab Work==
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We are a bit short handed in the lab this week, because most team members have exams. Considering the fine weather outside, it is not bad to have a slow week. All the transformations of [http://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010 yesterday] containing BioBricks form the distribution plate gave colonies. We pick a colony of every plate and grow them overnight in 50 mL LB medium containing Ampicillin.
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We are a bit short handed in the lab this week, because most team members have exams. Considering the fine weather outside, it is not bad to have a slow week.  
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<h4>BioBrick stocks</h4>
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All the transformations of [http://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010 yesterday] containing BioBricks form the distribution plate gave colonies. We pick a colony of every plate and grow them overnight in 50 mL LB medium containing Ampicillin.
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<h4>''Pseudomonas Putida'' GPO1</h4>
Today we cultivated ''Pseudomonas Putida'' GPO1 on different substrates to test on what carbon source it can grow:
Today we cultivated ''Pseudomonas Putida'' GPO1 on different substrates to test on what carbon source it can grow:
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5. E2 medium + 10% octane
5. E2 medium + 10% octane
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6. LB medium + 10% cyclohexane
 

Revision as of 18:14, 17 July 2010

Lab Work

We are a bit short handed in the lab this week, because most team members have exams. Considering the fine weather outside, it is not bad to have a slow week.

BioBrick stocks

All the transformations of yesterday containing BioBricks form the distribution plate gave colonies. We pick a colony of every plate and grow them overnight in 50 mL LB medium containing Ampicillin.

Pseudomonas Putida GPO1

Today we cultivated Pseudomonas Putida GPO1 on different substrates to test on what carbon source it can grow:

1. E2 medium without carbon source

2. E2 medium + 1% LB

3. E2 medium + 10% LB

4. E2 medium + 1% octane

5. E2 medium + 10% octane