Team:TU Delft/27 September 2010 content


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Alkane degradation

A colony PCR was run over night, and analyzed on gel today. Of the 022 and 023 none seemed correct, but 014 showed 2 positives (#2 and #7).

Colony PCR Gel (20100927_PCR) (insert here)

Because the height of 014 was not too clear on the previous gel, the same sample was loaded again on a new gel to double check the height. The height seemed correct so the plasmids were isolated and transformed to K12 immediately.

Colony PCR gel 2 (20100927_PCR2)

Salt tolerance

After looking at the results we can see some effects of bbc1, but we'll need to redo it for more salt concentrations.