Team:TU Delft/27 September 2010 content

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(New page: =Alkane degradation= A colony PCR was run over night, and analyzed on gel today. Of the 022 and 023 none seemed correct, but 014 showed 2 positives (#2 and #7). Colony PCR Gel (20100927_P...)
(Alkane degradation)
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Colony PCR Gel (20100927_PCR) (insert here)
Colony PCR Gel (20100927_PCR) (insert here)
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Because the height of 014 was not too clear on the previous gel, the same sample was loaded again on a new gel to double check the height. The height seemed correct so the plasmids were isolated and transformed to K12 immediately.
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Colony PCR gel 2 (20100927_PCR2)

Revision as of 08:33, 13 October 2010

Alkane degradation

A colony PCR was run over night, and analyzed on gel today. Of the 022 and 023 none seemed correct, but 014 showed 2 positives (#2 and #7).

Colony PCR Gel (20100927_PCR) (insert here)

Because the height of 014 was not too clear on the previous gel, the same sample was loaded again on a new gel to double check the height. The height seemed correct so the plasmids were isolated and transformed to K12 immediately.

Colony PCR gel 2 (20100927_PCR2)