"
Team:TU Delft/27 September 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: =Alkane degradation= A colony PCR was run over night, and analyzed on gel today. Of the 022 and 023 none seemed correct, but 014 showed 2 positives (#2 and #7). Colony PCR Gel (20100927_P...) |
Ravandervalk (Talk | contribs) (→Alkane degradation) |
||
| (One intermediate revision not shown) | |||
| Line 3: | Line 3: | ||
Colony PCR Gel (20100927_PCR) (insert here) | Colony PCR Gel (20100927_PCR) (insert here) | ||
| + | |||
| + | Because the height of 014 was not too clear on the previous gel, the same sample was loaded again on a new gel to double check the height. The height seemed correct so the plasmids were isolated and transformed to K12 immediately. | ||
| + | |||
| + | Colony PCR gel 2 (20100927_PCR2) | ||
| + | |||
| + | |||
| + | =Salt tolerance= | ||
| + | |||
| + | After looking at the results we can see some effects of bbc1, but we'll need to redo it for more salt concentrations. | ||
Latest revision as of 09:45, 15 October 2010
Alkane degradation
A colony PCR was run over night, and analyzed on gel today. Of the 022 and 023 none seemed correct, but 014 showed 2 positives (#2 and #7).
Colony PCR Gel (20100927_PCR) (insert here)
Because the height of 014 was not too clear on the previous gel, the same sample was loaded again on a new gel to double check the height. The height seemed correct so the plasmids were isolated and transformed to K12 immediately.
Colony PCR gel 2 (20100927_PCR2)
Salt tolerance
After looking at the results we can see some effects of bbc1, but we'll need to redo it for more salt concentrations.
