Team:TU Delft/24 September 2010 content

From 2010.igem.org

(Difference between revisions)
(Alkane degradation)
(Alkane degradation)
 
Line 2: Line 2:
Today we did a DLT to make 014 (011+028=>alkB2 gene cluster), 022 (017+026=>ladA, ADH and ALDH) and 023 (027+026, same as 022 but with different promoter). The ligation mixes were used to transform commercial TOP10 cells.
Today we did a DLT to make 014 (011+028=>alkB2 gene cluster), 022 (017+026=>ladA, ADH and ALDH) and 023 (027+026, same as 022 but with different promoter). The ligation mixes were used to transform commercial TOP10 cells.
 +
No encouraging results from the resting cell assays :-( We are going to use other protocol. Maybe cell disruption and NADH measurements...
=Salt tolerance=  
=Salt tolerance=  
We ran an innitial test of our salt tolerance biobrick.
We ran an innitial test of our salt tolerance biobrick.
We'll have the results on monday
We'll have the results on monday

Latest revision as of 13:22, 27 October 2010

Alkane degradation

Today we did a DLT to make 014 (011+028=>alkB2 gene cluster), 022 (017+026=>ladA, ADH and ALDH) and 023 (027+026, same as 022 but with different promoter). The ligation mixes were used to transform commercial TOP10 cells.

No encouraging results from the resting cell assays :-( We are going to use other protocol. Maybe cell disruption and NADH measurements...

Salt tolerance

We ran an innitial test of our salt tolerance biobrick. We'll have the results on monday