Team:TU Delft/23 June 2010 content


Revision as of 08:55, 6 July 2010 by Ptmvanboheemen (Talk | contribs)

Lab Work

Ligations were performed using the digested BioBricks obtained in the early morning. We following ligation was performed:

Componet Sample
I10341 3 μL
J611XX 4 μL
pSB3C5 1.5 μL
Ligation Buffer 3 μL
T4 ligase 1 μL
H20 15 μL

We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.