Team:TU Delft/23 June 2010 content

From 2010.igem.org

(Difference between revisions)
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Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
-
|'''-'''
+
|Componet
-
|'''μL'''
+
|Sample
|-
|-
|I10341
|I10341
-
|3
+
|3 μL
|-
|-
|J611XX
|J611XX
-
|4
+
|4 μL
|-
|-
|pSB3C5
|pSB3C5
-
|1.5
+
|1.5 μL
|-
|-
|Ligation Buffer
|Ligation Buffer
-
|3
+
|3 μL
|-
|-
|T4 ligase
|T4 ligase
-
|1 unit
+
|1 μL
|-
|-
|H20
|H20
-
|15
+
|15 μL
|}
|}
 +
 +
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added.
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added.
The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.
The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.

Revision as of 11:49, 5 July 2010

LAB WORK

Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:

Componet Sample
I10341 3 μL
J611XX 4 μL
pSB3C5 1.5 μL
Ligation Buffer 3 μL
T4 ligase 1 μL
H20 15 μL


We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.