Team:TU Delft/23 June 2010 content

From 2010.igem.org

(Difference between revisions)
(Characterization of Anderson RBS sequences)
Line 2: Line 2:
==Characterization of Anderson RBS sequences==
==Characterization of Anderson RBS sequences==
-
[[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight [http://2010.igem.org/Team:TU_Delft#/blog?blog=22_June_2010 digested BioBricks]. The following ligation reactions was performed:  
+
[[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight [http://2010.igem.org/Team:TU_Delft#page=pages/blog?blog=22_June_2010 digested BioBricks]. The following ligation reactions was performed:  
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"

Revision as of 12:26, 3 August 2010

Lab work

Characterization of Anderson RBS sequences

Ligations were performed using the overnight digested BioBricks. The following ligation reactions was performed:

# BioBrick Fragment 1 Fragment 2 Recipient vector
1 4 μL μL ‘E–J1100–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
2 4 μL μL ‘E–J1101–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
3 4 μL μL ‘E–J1107–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
4 4 μL μL ‘E–J1117–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
5 4 μL μL ‘E–J1127–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E-linear pSB1T3–P’
6 negative control - 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’

We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The mixtures were incubated overnight at 16 °C.