Team:TU Delft/23 June 2010 content

From 2010.igem.org

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==Lab work==
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=Lab work=
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<h4>Characterization of Anderson RBS sequences</h4>
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==Characterization of Anderson RBS sequences==
[[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight [http://2010.igem.org/Team:TU_Delft#/blog?blog=22_June_2010 digested BioBricks]. The following ligation reactions was performed:  
[[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight [http://2010.igem.org/Team:TU_Delft#/blog?blog=22_June_2010 digested BioBricks]. The following ligation reactions was performed:  

Revision as of 19:18, 2 August 2010

Lab work

Characterization of Anderson RBS sequences

Ligations were performed using the overnight digested BioBricks. The following ligation reactions was performed:

# BioBrick Fragment 1 Fragment 2 Recipient vector
1 4 μL μL ‘E–J1100–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
2 4 μL μL ‘E–J1101–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
3 4 μL μL ‘E–J1107–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
4 4 μL μL ‘E–J1117–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’
5 4 μL μL ‘E–J1127–S’ 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E-linear pSB1T3–P’
6 negative control - 3 μL μL ‘X–I10341–P’ 1.5 μL ‘E–linear pSB1T3–P’

We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The mixtures were incubated overnight at 16 °C.