Team:TU Delft/23 June 2010 content

From 2010.igem.org

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'''LAB WORK'''
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===Lab Work===
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Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
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[[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the digested BioBricks obtained in the early morning. We following ligation was performed:
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
|Componet
|Componet

Revision as of 08:55, 6 July 2010

Lab Work

Ligations were performed using the digested BioBricks obtained in the early morning. We following ligation was performed:

Componet Sample
I10341 3 μL
J611XX 4 μL
pSB3C5 1.5 μL
Ligation Buffer 3 μL
T4 ligase 1 μL
H20 15 μL


We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.