Team:TU Delft/23 June 2010 content

From 2010.igem.org

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Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
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{| style="color:green; background-color:#ffffcc;" cellpadding="10" cellspacing="0" border="1"
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|I10341
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We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added.
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added.
The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.
The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.

Revision as of 11:46, 5 July 2010

LAB WORK

Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:

- μL
I10341 3
J611XX 4
pSB3C5 1.5
Ligation Buffer 3
T4 ligase 1 unit
H20 15

We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.