Team:TU Delft/23 June 2010
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Revision as of 14:19, 24 June 2010
Lab workCharacterization of Anderson RBS sequencesLigations were performed using the overnight digested BioBricks. The following ligation reactions was performed:
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The mixtures were incubated overnight at 16 °C. Media and solutionsBy Hugo The recipe is as follows:
WARNING: THE POWDERS ARE ELECTROSTATIC. WEAR GLOVES AND CLEAN ALL THE PLACE WITH ALCOHOL (70% v/v)... SERIOUSLY, THE BLUE STUFF IS EVERYWHERE!!! Return to the calendar |
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