Team:TU Delft/23 July 2010 content

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Alkane Degradation

With a number of yesterday's colonies a digestion was done to check if they contain the desired insert. The table below describes the digestion procedure, as well as the gel lane description.

# Contents Enzyme 1 Enzyme 2 Buffer BSA Expected size(s) (bp)
1 Smartladder (5μl) n/a
2 007T (2) 0.5 μl EcoRI 0.5 μl PstI 3 (Biolabs) no 1341, 2455
3 008T (4) 0.5 μl EcoRI 0.5 μl PstI 3 (Biolabs) no 276, 2455
4 009T (6) 0.5 μl EcoRI 0.5 μl PstI 3 (Biolabs) no 285, 2455
5 009T (7) 0.5 μl EcoRI 0.5 μl PstI 3 (Biolabs) no 285, 2455
6 010T (9) 0.5 μl EcoRI 0.5 μl PstI 3 (Biolabs) no 1382, 2455
7 017T (14) 0.5 μl EcoRI 0.5 μl PstI 3 (Biolabs) no 855, 2455
8 Empty
9 007T (2) 1 μl PstI 1 μl PvuII H (Boehringer) no 2529,983,251
10 008T (4) 1 μl AatII 0.5 μl KpnI A (Boehringer) 1 μl 271, 2427
11 009T (6) 0.5 μl KpnI 1 μl NruI B (Boehringer) 1 μl 598, 2109
12 009T (7) 0.5 μl KpnI 1 μl NruI B (Boehringer) 1 μl 598, 2109
13 010T (9) 1 μl EarI 0.5 μl PstI 1 (Biolabs) no 1075, 2729
14 017T (14) 0.5 μl AdhI 0.5 μl SpeI 4 (Biolabs) no 428, 2849
15 EZ load (ladder) n/a
Digestion check