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From 2010.igem.org

Alkane Degradation

With a number of yesterday's colonies a digestion was done to check if they contain the desired insert. The table below describes the digestion procedure, as well as the gel lane description.

|# |Contents |Enzyme 1 |Enzyme 2 |Buffer |BSA |Expected size(s) |- |1 |Smartladder (5μl) | | | | |n/a |- |2 |007T (2) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |3 |008T (4) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |4 |009T (6) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |5 |009T (7) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |6 |010T (9) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |7 |017T (14) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |-

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