Team:TU Delft/23 July 2010 content

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(Difference between revisions)
(New page: ==Alkane Degradation== With a number of [https://2010.igem.org/Team:TU_Delft#/blog?blog=22_July_2010 yesterday's]] colonies [[Image:TUDelft_Digestion_Kira_23-07.png|200px|thumb|left|Dige...)
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==Alkane Degradation==
==Alkane Degradation==
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With a number of [https://2010.igem.org/Team:TU_Delft#/blog?blog=22_July_2010 yesterday's]] colonies
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With a number of [https://2010.igem.org/Team:TU_Delft#/blog?blog=22_July_2010 yesterday's] colonies a digestion was done to check if they contain the desired insert. The table below describes the digestion procedure, as well as the gel lane description.
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|'''#'''
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|'''Contents'''
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|'''Enzyme 1'''
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|'''Enzyme 2'''
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|'''Buffer'''
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|'''BSA'''
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|'''Expected size(s)'''
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|-
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|1
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|Smartladder (5μl)
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|
 +
|
 +
|
 +
|
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|n/a
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|-
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|2
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|007T (2)
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|0.5 μl EcoRI
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|0.5 μl PstI
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|Buffer 3 (Biolabs)
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|no
 +
|-
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|3
 +
|008T (4)
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|0.5 μl EcoRI
 +
|0.5 μl PstI
 +
|Buffer 3 (Biolabs)
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|no
 +
|-
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|4
 +
|009T (6)
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|0.5 μl EcoRI
 +
|0.5 μl PstI
 +
|Buffer 3 (Biolabs)
 +
|no
 +
|-
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|5
 +
|009T (7)
 +
|0.5 μl EcoRI
 +
|0.5 μl PstI
 +
|Buffer 3 (Biolabs)
 +
|no
 +
|-
 +
|6
 +
|010T (9)
 +
|0.5 μl EcoRI
 +
|0.5 μl PstI
 +
|Buffer 3 (Biolabs)
 +
|no
 +
|-
 +
|7
 +
|017T (14)
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|0.5 μl EcoRI
 +
|0.5 μl PstI
 +
|Buffer 3 (Biolabs)
 +
|no
 +
|-
 +
 
 +
 
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|}
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[[Image:TUDelft_Digestion_Kira_23-07.png|200px|thumb|left|Digestion check]]
[[Image:TUDelft_Digestion_Kira_23-07.png|200px|thumb|left|Digestion check]]

Revision as of 10:41, 26 July 2010

Alkane Degradation

With a number of yesterday's colonies a digestion was done to check if they contain the desired insert. The table below describes the digestion procedure, as well as the gel lane description.

|# |Contents |Enzyme 1 |Enzyme 2 |Buffer |BSA |Expected size(s) |- |1 |Smartladder (5μl) | | | | |n/a |- |2 |007T (2) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |3 |008T (4) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |4 |009T (6) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |5 |009T (7) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |6 |010T (9) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |- |7 |017T (14) |0.5 μl EcoRI |0.5 μl PstI |Buffer 3 (Biolabs) |no |-


|}



Digestion check
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