"
Team:TU Delft/22 September 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: ==Emulsifier== The K12 cultures were grown over night on M9. We tried to do 2 experiments today. One for getting samples for a protein gel and another one for samples for the emulsifier as...) |
(→Protein gel) |
||
| Line 34: | Line 34: | ||
| | | | ||
|} | |} | ||
| + | |||
| + | 0.5 mL samples were taken, spinned down, and the sup and pellet were stored at -20. | ||
Revision as of 12:11, 24 September 2010
Emulsifier
The K12 cultures were grown over night on M9. We tried to do 2 experiments today. One for getting samples for a protein gel and another one for samples for the emulsifier assay.
Protein gel
One 50 mL tube with 10 mL M9 + Glucose was inocculated with K12 that was transformed with 206. Once the OD600 was 0.6 the protein production was induced by adding IPTG to a final concentration of 1 mM. Samples were taken every hour, for 4 hours and over night.
| # | Time point | OD600 |
| 1 | 0 | 0.6 |
| 2 | 1 | 1.0 |
| 3 | 2 | 1.16 |
| 4 | 3 | 1.17 |
| 5 | 4 | |
| 6 | 0/n |
0.5 mL samples were taken, spinned down, and the sup and pellet were stored at -20.
