Team:TU Delft/22 September 2010 content

From 2010.igem.org

(Difference between revisions)
(→Protein gel)
(→Emulsifier)
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0.5 mL samples were taken, spinned down, and the sup and pellet were stored at -20.
0.5 mL samples were taken, spinned down, and the sup and pellet were stored at -20.
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===Emulsifier assay===
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The experiment was done in triplo. 50 mL tubes were filled with 10 mL M9 + glucose and inocculated. Once OD600 was 0.6 the protein production was induced by adding IPTG to a final concentration of 1 mM.
 +
 +
1 mL samples were taken at T=0, 2 and 4.
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 +
The samples were immediately subjected to the emulsifier assay with Sudan II.
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 +
T=0
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{|
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|'''#'''
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|'''Culture'''
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|'''Sudan II'''
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|'''Absorption 493 nm'''
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|-
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|1
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|1
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| -
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|0.981
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|-
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|2
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|1
 +
| +
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|1.211
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|-
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|3
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|2
 +
| -
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|0.985
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|-
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|4
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|2
 +
| +
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|1.266
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|-
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|5
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|3
 +
| -
 +
|0.985
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|-
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|6
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|3
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| -
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|1.243
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|-
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|}

Revision as of 12:17, 24 September 2010

Emulsifier

The K12 cultures were grown over night on M9. We tried to do 2 experiments today. One for getting samples for a protein gel and another one for samples for the emulsifier assay.

Protein gel

One 50 mL tube with 10 mL M9 + Glucose was inocculated with K12 that was transformed with 206. Once the OD600 was 0.6 the protein production was induced by adding IPTG to a final concentration of 1 mM. Samples were taken every hour, for 4 hours and over night.

# Time point OD600
1 0 0.6
2 1 1.0
3 2 1.16
4 3 1.17
5 4
6 0/n

0.5 mL samples were taken, spinned down, and the sup and pellet were stored at -20.

Emulsifier assay

The experiment was done in triplo. 50 mL tubes were filled with 10 mL M9 + glucose and inocculated. Once OD600 was 0.6 the protein production was induced by adding IPTG to a final concentration of 1 mM.

1 mL samples were taken at T=0, 2 and 4.

The samples were immediately subjected to the emulsifier assay with Sudan II.

T=0

# Culture Sudan II Absorption 493 nm
1 1 - 0.981
2 1 + 1.211
3 2 - 0.985
4 2 + 1.266
5 3 - 0.985
6 3 - 1.243
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