Team:TU Delft/22 July 2010 content
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(New page: ==Alkane degradation== There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen...) |
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==Alkane degradation== | ==Alkane degradation== | ||
There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a [[Team:TU_Delft/protocols/colony PCR|colony PCR]]. | There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a [[Team:TU_Delft/protocols/colony PCR|colony PCR]]. | ||
+ | |||
+ | [[Image:TUDelft_scPCR_22-07.png|400px|thumb|left|1% Agarose gel of SC PCR samples]] | ||
+ | |||
+ | |'''#''' | ||
+ | |'''Description''' | ||
+ | |'''Expected length (bp)''' | ||
+ | |- | ||
+ | |1 | ||
+ | |[[https://2010.igem.org/Image:TU_Delft_SmartLadder.jpg SmartLadder]] (5 μL) | ||
+ | |n/a | ||
+ | |- | ||
+ | |2 | ||
+ | |007T | ||
+ | |1616 | ||
+ | |- | ||
+ | |3 | ||
+ | |007T | ||
+ | |1616 | ||
+ | |- | ||
+ | |4 | ||
+ | |007T | ||
+ | |1616 | ||
+ | |- | ||
+ | |5 | ||
+ | |008T | ||
+ | |551 | ||
+ | |- | ||
+ | |6 | ||
+ | |008T | ||
+ | |551 | ||
+ | |- | ||
+ | |7 | ||
+ | |009T | ||
+ | |560 | ||
+ | |- | ||
+ | |8 | ||
+ | |009T | ||
+ | |560 | ||
+ | |- | ||
+ | |9 | ||
+ | |009T | ||
+ | |560 | ||
+ | |- | ||
+ | |10 | ||
+ | |010T | ||
+ | |1657 | ||
+ | |- | ||
+ | |11 | ||
+ | |010T | ||
+ | |1657 | ||
+ | |- | ||
+ | |12 | ||
+ | |010T | ||
+ | |1657 | ||
+ | |- | ||
+ | |13 | ||
+ | |017T | ||
+ | |1130 | ||
+ | |- | ||
+ | |14 | ||
+ | |017T | ||
+ | |1130 | ||
+ | |- | ||
+ | |15 | ||
+ | |017T | ||
+ | |1130 | ||
+ | |- | ||
+ | |16 | ||
+ | |018T | ||
+ | |1874 | ||
+ | |- | ||
+ | |17 | ||
+ | |Red colony | ||
+ | |n/a | ||
+ | |} |
Revision as of 07:37, 23 July 2010
Alkane degradation
There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.
|# |Description |Expected length (bp) |- |1 |[SmartLadder] (5 μL) |n/a |- |2 |007T |1616 |- |3 |007T |1616 |- |4 |007T |1616 |- |5 |008T |551 |- |6 |008T |551 |- |7 |009T |560 |- |8 |009T |560 |- |9 |009T |560 |- |10 |010T |1657 |- |11 |010T |1657 |- |12 |010T |1657 |- |13 |017T |1130 |- |14 |017T |1130 |- |15 |017T |1130 |- |16 |018T |1874 |- |17 |Red colony |n/a |}