Team:TU Delft/21 June 2010 content

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Lab work

Characterization of Anderson RBS sequences

Restriction digestion of plasmid backbone pSB1T3 using Buffer H of Roche.

Digestion reaction Used Buffer Needed fragment
1,0 μg pSB1T3 + 1 U/μL EcoRI + 1 U/μL PstI Buffer H (Roche) ‘E – ---- – P’

The digested pSB1T3 was cut from gel and isolate the band. However, the gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.