Team:TU Delft/21 June 2010 content


Revision as of 13:37, 24 June 2010 by Ebrinkman (Talk | contribs)

We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.

Lab work

Restriction digestion of plasmid backbone pSB1T3:

pSB1T3 (~1,0 μg) 2,5 μL
Buffer H (Roche) 2,0 μL
EcoRI 1,0 μL
PstI 1,0 μL
H2O 13,5 μL

Cut digested pSB1T3 from gel and isolate the band.