Team:TU Delft/21 June 2010 content

From 2010.igem.org

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===Lab work===
===Lab work===
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[[Team:TU_Delft/protocols/restriction_enzyme_digestion|Restriction digestion]] of plasmid backbone pSB1T3:
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[[Team:TU_Delft/protocols/restriction_enzyme_digestion|Restriction digestion]] of plasmid backbone pSB1T3 (~1,0 μg) with restriction enzymes EcoRI (1 U/μg) and PstI (1 U/μg). The digested pSB1T3 was cut from gel and isolate the band.  
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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However, the gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
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|pSB1T3 (~1,0 μg)
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|2,5 μL
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|Buffer H (Roche)
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|2,0 μL
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|EcoRI (1 U/μg)
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|1,0 μL
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|PstI (1 U/μg)
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|1,0 μL
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|H2O
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|13,5 μL
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Cut digested pSB1T3 from gel and isolate the band.
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We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
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Revision as of 18:16, 12 July 2010

Lab work

Restriction digestion of plasmid backbone pSB1T3 (~1,0 μg) with restriction enzymes EcoRI (1 U/μg) and PstI (1 U/μg). The digested pSB1T3 was cut from gel and isolate the band. However, the gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.

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