Team:TU Delft/21 June 2010 content

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(Lab work)
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We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
 
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===Lab work===
===Lab work===
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Restriction digestion of plasmid backbone pSB1T3:
 
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[[Team:TU_Delft/protocols/restriction_enzyme_digestion|Restriction digestion]] of plasmid backbone pSB1T3:
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
|pSB1T3 (~1,0 μg)
|pSB1T3 (~1,0 μg)
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Cut digested pSB1T3 from gel and isolate the band.
Cut digested pSB1T3 from gel and isolate the band.
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We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.

Revision as of 08:46, 6 July 2010

Lab work

Restriction digestion of plasmid backbone pSB1T3:

pSB1T3 (~1,0 μg) 2,5 μL
Buffer H (Roche) 2,0 μL
EcoRI (1 U/μg) 1,0 μL
PstI (1 U/μg) 1,0 μL
H2O 13,5 μL

Cut digested pSB1T3 from gel and isolate the band.

We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.