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Team:TU Delft/21 June 2010 content
From 2010.igem.org
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Restriction digestion of plasmid backbone pSB1T3: | Restriction digestion of plasmid backbone pSB1T3: | ||
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|pSB1T3 (~1,0 μg) | |pSB1T3 (~1,0 μg) | ||
|2,5 μL | |2,5 μL | ||
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|13,5 μL | |13,5 μL | ||
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| + | Cut digested pSB1T3 from gel and isolate the band. | ||
Revision as of 13:30, 24 June 2010
We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
Lab work
Restriction digestion of plasmid backbone pSB1T3:
| pSB1T3 (~1,0 μg) | 2,5 μL |
| Buffer H (Roche) | 2,0 μL |
| EcoRI | 1,0 μL |
| PstI | 1,0 μL |
| H2O | 13,5 μL |
Cut digested pSB1T3 from gel and isolate the band.
