Team:TU Delft/20 July 2010 content
From 2010.igem.org
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'''BioBrick production continued''' | '''BioBrick production continued''' | ||
- | A [[Team:TU_Delft/protocols/transformation|transformation]] of [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 yesterday's] ligation products was done in | + | A [[Team:TU_Delft/protocols/transformation|transformation]] of [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 yesterday's] ligation products was done in TOP10 competent cells, and grown on LB-TET plates overnight. Hopefully we will have some colonies tomorrow. |
+ | |||
+ | ==Characterization of Anderson RBS sequences== | ||
+ | <h5>Assembly of positive control</h5> | ||
+ | Single colony PCRs were performed on the transformants obtained yesterday. These were loaded onto 1% agarose gel (see gel below) | ||
+ | |||
+ | [[Image:TU_Delft_ScPCR-digPCR-20Jul10.png|500px|thumb|left|scPCR]] | ||
+ | |||
+ | Lane description: | ||
+ | {|style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
+ | |'''#''' | ||
+ | |'''Description''' | ||
+ | |'''Expected Length (bp)''' | ||
+ | |'''Remarks''' | ||
+ | |- | ||
+ | |1 | ||
+ | |SmartLadder (5 μL) | ||
+ | |Varying | ||
+ | |Visible | ||
+ | |- | ||
+ | |2 | ||
+ | |J23100-B0030-I13401 colony 1 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |3 | ||
+ | |J23100-B0030-I13401 colony 2 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |4 | ||
+ | |J23100-B0030-I13401 colony 3 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |5 | ||
+ | |J23100-B0030-I13401 colony 4 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |6 | ||
+ | |J23100-B0030-I13401 colony 5 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |7 | ||
+ | |I13401 ligation control colony 1 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |8 | ||
+ | |I13401 ligation control colony 2 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |9 | ||
+ | |I13401 ligation control colony 3 | ||
+ | | | ||
+ | |Not visible | ||
+ | |- | ||
+ | |10 | ||
+ | |I13401 digestion control colony 1 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |11 | ||
+ | |I13401 digestion control colony 2 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |12 | ||
+ | |K081005 colony 1 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |13 | ||
+ | |K081005 colony 2 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |14 | ||
+ | |K081005 colony 3 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |15 | ||
+ | |BioRad EZ Load | ||
+ | |Varying | ||
+ | |Visible | ||
+ | |- | ||
+ | |16 | ||
+ | |PCR product of E0240 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |17 | ||
+ | |PCR product of E0240 | ||
+ | | | ||
+ | |Visible | ||
+ | |- | ||
+ | |18 | ||
+ | |PCR product of E0240 | ||
+ | | | ||
+ | |Visible | ||
+ | |} |
Revision as of 16:08, 21 July 2010
Alkane degradation
BioBrick production continued
A transformation of yesterday's ligation products was done in TOP10 competent cells, and grown on LB-TET plates overnight. Hopefully we will have some colonies tomorrow.
Characterization of Anderson RBS sequences
Assembly of positive control
Single colony PCRs were performed on the transformants obtained yesterday. These were loaded onto 1% agarose gel (see gel below)
Lane description:
# | Description | Expected Length (bp) | Remarks |
1 | SmartLadder (5 μL) | Varying | Visible |
2 | J23100-B0030-I13401 colony 1 | Not visible | |
3 | J23100-B0030-I13401 colony 2 | Not visible | |
4 | J23100-B0030-I13401 colony 3 | Not visible | |
5 | J23100-B0030-I13401 colony 4 | Not visible | |
6 | J23100-B0030-I13401 colony 5 | Not visible | |
7 | I13401 ligation control colony 1 | Not visible | |
8 | I13401 ligation control colony 2 | Visible | |
9 | I13401 ligation control colony 3 | Not visible | |
10 | I13401 digestion control colony 1 | Visible | |
11 | I13401 digestion control colony 2 | Visible | |
12 | K081005 colony 1 | Visible | |
13 | K081005 colony 2 | Visible | |
14 | K081005 colony 3 | Visible | |
15 | BioRad EZ Load | Varying | Visible |
16 | PCR product of E0240 | Visible | |
17 | PCR product of E0240 | Visible | |
18 | PCR product of E0240 | Visible |