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Team:TU Delft/20 July 2010 content
From 2010.igem.org
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Revision as of 07:28, 2 August 2010
Contents |
Juggling an Diabolo tricks!
From all the pipetting that we do in the lab, our fingers and wrists are a bit tense. After some hard thinking, we found a perfect way to relax them…..by juggling and performing diabolo tricks! So now, in between the incubation times of our experiments the juggle balls are flying around in our iGEM room.
Lab work
Competent cells
Eva made another batch of competent cells
Alkane degradation
BioBrick production continued
A transformation of yesterday's ligation products was done in TOP10 competent cells, and grown on LB-Tetracycline plates overnight. Hopefully we will have some colonies tomorrow.
Characterization of Anderson RBS sequences
Assembly of reference construct
Single colony PCRs were performed on the transformants obtained yesterday. These were loaded onto 1% agarose gel (see gel below)
Lane description:
| # | Description | Expected Length (bp) | Primers | Status | Remarks |
| 1 | SmartLadder | n/a | n/a | n/a | |
| 2 | transformant #1 of ligation mix: J23100-B0030-I13401 | G00101 + G00101 | ✗ | ||
| 3 | transformant #2 of ligation mix: J23100-B0030-I13401 | G00101 + G00101 | ✗ | ||
| 4 | transformant #3 of ligation mix: J23100-B0030-I13401 | G00101 + G00101 | ✗ | ||
| 5 | transformant #4 of ligation mix: J23100-B0030-I13401 | G00101 + G00101 | ✗ | ||
| 6 | transformant #5 of ligation mix: J23100-B0030-I13401 | G00101 + G00101 | ✗ | ||
| 7 | transformant #1 of ligation mix: I13401 ligation control | G00101 + G00101 | ✗ | ||
| 8 | transformant #2 of ligation mix: I13401 ligation control | G00101 + G00101 | ✓ | ||
| 9 | transformant #3 of ligation mix: I13401 ligation control | G00101 + G00101 | ✓ | ||
| 10 | transformant #1 of ligation mix: I13401 digestion control | G00101 + G00101 | ✓ | ||
| 11 | transformant #2 of ligation mix: I13401 digestion control | G00101 + G00101 | ✓ | ||
| 12 | transformant #1 of K081005 | G00101 + G00101 | ✓ | ||
| 13 | transformant #2 of K081005 | G00101 + G00101 | ✓ | ||
| 14 | transformant #3 of K081005 | G00101 + G00101 | ✓ | ||
| 15 | BioRad EZ Load | n/a | n/a | n/a | |
| 16 | PCR product of E0240 | 1114 | G00101 + G00101 | ✓ | |
| 17 | PCR product of E0240 | 1114 | G00101 + G00101 | ✓ | |
| 18 | PCR product of E0240 | 1114 | G00101 + G00101 | ✓ |
