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From 2010.igem.org

Lab work

Alkane degradation

Biobricks in production:

Digestion reactions:

#	Digestion reaction	Used Buffer	Needed fragment
1	2 μg J61100 + EcoRI + SpeI	NEBuffer 2	‘E – J61100 – S’
2	2 μg J61100 + EcoRI + SpeI	NEBuffer 2	‘E – J61100 – S’
3	1 μg rubR + EcoRI + SpeI	NEBuffer 2	‘E – rubR– S’
4	1 μg J61101 + EcoRI + SpeI	NEBuffer 2	‘E – J61101– S’
5	1 μg J61107 + EcoRI + SpeI	NEBuffer 2	‘E – J61107– S’
6	1μg alkB2 + Xbal + PstI	NEBuffer 2	‘X – alkB2 – P’
7	1 μg rubA3 + Xbal + PstI	NEBuffer 2	‘X – rubA3 – P’
8	1 μg rubA4 + Xbal + PstI	NEBuffer 2	‘X – rubA4 – P’
9	1 μg B0015 + Xbal + PstI	NEBuffer 2	‘X – B0015 – P’
10	1 μg ladA + Xbal + PstI	NEBuffer 2	‘X – ladA – P’
11	1 μg ADH + Xbal + PstI	NEBuffer 2	‘X – ADH – P’
12	1 μg ALDH + Xbal + PstI	NEBuffer 2	‘X – ALDH – P’
13	3 μg pSB1T3 + EcoRI + PstI	NEBuffer 2	‘E – ALDH – P’