"
Team:TU Delft/18 October 2010 content
From 2010.igem.org
(New page: ==Lab work== The deadline is now really closing in. Everyone is super busy in the lab to reproduce as much data as possible to make our results ever prettier. So, less time is spend on blo...) |
(→Emulsifier) |
||
| Line 3: | Line 3: | ||
==Emulsifier== | ==Emulsifier== | ||
| - | We tried to isolate the emulsifier proteins from the pellets we made last friday. It was a bit disappointing, but expected, that the total protein yield was lower than last time. This is probably caused by the fact that we stopped the growth of the culture before it reached the stationairy phase at OD600 of 0.6. The total volume per sample was 1 mL. | + | We tried to isolate the emulsifier proteins from the pellets we made last friday. It was a bit disappointing, but expected, that the total protein yield was lower than last time. This is probably caused by the fact that we stopped the growth of the culture before it reached the stationairy phase at OD600 of 0.6. The total finale volume per sample was 1 mL. |
The rough protien yields were determined by Bradford analysis: | The rough protien yields were determined by Bradford analysis: | ||
Revision as of 15:50, 18 October 2010
Lab work
The deadline is now really closing in. Everyone is super busy in the lab to reproduce as much data as possible to make our results ever prettier. So, less time is spend on blogging :)
Emulsifier
We tried to isolate the emulsifier proteins from the pellets we made last friday. It was a bit disappointing, but expected, that the total protein yield was lower than last time. This is probably caused by the fact that we stopped the growth of the culture before it reached the stationairy phase at OD600 of 0.6. The total finale volume per sample was 1 mL.
The rough protien yields were determined by Bradford analysis:
| # | Description | [Protein](mg/ml) |
| 1 | AlnA 37 C | 1.55 |
| 2 | AlnA 30 C | 2.29 |
| 3 | Control 37 C | 1.78 |
| 4 | Control 30 C | 2.42 |
So it looks like Esengul was right about the higher IPTG induction at lower growth temperatures.
The samples were used in the emulsifier assay, to determine their emulsifying capacity. I used 0.75 mg of protein in each sample. This is the first draft of the data analysis:
