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Team:TU Delft/18 June 2010 content
From 2010.igem.org
Contents |
Cleaning
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
We also booked a hotel in Boston!
Lab work
Emulsifier
Pieter worked on the emulsifying activity assay. Four samples were tested:
1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
| # | Amount of SDS (%) | Hexalkane (mL) |
| 1 | 0 | 0 |
| 2 | 0 | 0.02 |
| 3 | 0.01 | 0.02 |
| 4 | 1 | 0.02 |
2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.
3. Shake 1 hour with 200 strokes/min at 30°C
4. Measure absorbance OD600
| # | A600 |
| 1 | 0 |
| 2 | 0 |
| 3 | 0.4 |
| 4 | 0.2 |
Biobricks
By Hugo
I've got some colonies in the plates, however there's contamination with some yellow colonies :s Probably, we will have to prepare a new stock of competent cells.
