Team:TU Delft/18 June 2010 content

From 2010.igem.org

(Difference between revisions)

Revision as of 18:13, 12 July 2010

After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Pieter worked on the emulsifying activity assay. Four samples were tested:

1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.

Sample	Amount of SDS (%)	Hexalkane (mL)
1	0	0
2	0	0.02
3	0.01	0.02
4	1	0.02

2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.

3. Shake 1 hour with 200 strokes/min at 30°C

4. Measure absorbance OD600

Sample	A600
1	0
2	0
3	0.4
4	0.2

@@ Line 1: / Line 1: @@
-We had a nice slow day, finishing some small experiments and reorganizing our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
+After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
+We also booked a hotel in Boston!
-We also booked an hotel in Boston!
 ===Lab work===
-Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:
+Pieter worked on the emulsifying activity assay. Four samples were tested:
+.	Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
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-Tris buffer (pH 8) was added to a total volume of 7.5 ml.
-Incubate 1 hour at 30 °C at shaken at 200 rpm.
+.	Tris buffer (pH 8) was added to a total volume of 7.5 ml.
+.	Shake 1 hour with 200 strokes/min at 30°C
+.	Measure absorbance OD600
-Absorbance measured at 600 nm.
 {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"