Team:TU Delft/18 June 2010 content

From 2010.igem.org

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==Biobricks==
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By Hugo
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I've got some colonies in the plates, however there's contamination with some yellow colonies :s
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Probably, we will have to prepare a new stock of competent cells.

Latest revision as of 08:28, 26 August 2010

Contents

Cleaning

After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Emulsifier

Pieter worked on the emulsifying activity assay. Four samples were tested:

1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.

# Amount of SDS (%) Hexalkane (mL)
1 0 0
2 0 0.02
3 0.01 0.02
4 1 0.02

2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.

3. Shake 1 hour with 200 strokes/min at 30°C

4. Measure absorbance OD600

# A600
1 0
2 0
3 0.4
4 0.2

Biobricks

By Hugo

I've got some colonies in the plates, however there's contamination with some yellow colonies :s Probably, we will have to prepare a new stock of competent cells.