Team:TU Delft/18 August 2010 content

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Because the gels are inconclusive about biobrick 304 and 327 we decided to isolate the plasmids and send them to BaseClear for sequencing.

Revision as of 10:00, 27 August 2010

Strain Characterization

By Hugo

I'm working in some growth curves, today I tested a different amount of inoculum than yesterday.

Measurements!!!

Hour Time Glc Glc+CC6 C8
11:43 0h 0.015 0.014 0.007
14:54 3h 11' 0.042 0.052 0.066
16:00 4h 17' 0.099 0.081 0.086
17:00 5h 17' 0.196 0.128 0.142
18:00 6h 17' 0.355 0.215 0.184
19:00 7h 17' 0.555 0.341 0.275
9:43 22h 1.473 1.366 1.195
11:43 24h 1.459 1.360 1.398

Alkane Degradation parallel attempt

Yesterdays plates all contained colonies. But since this is a two way ligation, we expect a lot of religated backbones, so false positive colonies. That is why we performed a colony PCR.

We picked 5 colonies from each plate.

TU Delft 2010-08-18 Colony PCR.jpg

Lane description

# Description Expected size (bp) OK?
1 Smartladder n/a Yes
2-6 014 3000+ No
7-11 020 2100+ No
12-16 304 342 Maybe
17-21 327 310 Maybe

Because the gels are inconclusive about biobrick 304 and 327 we decided to isolate the plasmids and send them to BaseClear for sequencing.