Team:TU Delft/18 August 2010 content
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Revision as of 10:00, 27 August 2010
Strain Characterization
By Hugo
I'm working in some growth curves, today I tested a different amount of inoculum than yesterday.
Measurements!!!
Hour | Time | Glc | Glc+CC6 | C8 |
11:43 | 0h | 0.015 | 0.014 | 0.007 |
14:54 | 3h 11' | 0.042 | 0.052 | 0.066 |
16:00 | 4h 17' | 0.099 | 0.081 | 0.086 |
17:00 | 5h 17' | 0.196 | 0.128 | 0.142 |
18:00 | 6h 17' | 0.355 | 0.215 | 0.184 |
19:00 | 7h 17' | 0.555 | 0.341 | 0.275 |
9:43 | 22h | 1.473 | 1.366 | 1.195 |
11:43 | 24h | 1.459 | 1.360 | 1.398 |
Alkane Degradation parallel attempt
Yesterdays plates all contained colonies. But since this is a two way ligation, we expect a lot of religated backbones, so false positive colonies. That is why we performed a colony PCR.
We picked 5 colonies from each plate.
Lane description
# | Description | Expected size (bp) | OK? |
1 | Smartladder | n/a | Yes |
2-6 | 014 | 3000+ | No |
7-11 | 020 | 2100+ | No |
12-16 | 304 | 342 | Maybe |
17-21 | 327 | 310 | Maybe |
Because the gels are inconclusive about biobrick 304 and 327 we decided to isolate the plasmids and send them to BaseClear for sequencing.