Team:TU Delft/18 August 2010 content

From 2010.igem.org

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[[Image:TU Delft 2010-08-18 Colony PCR.jpg|500px]]
[[Image:TU Delft 2010-08-18 Colony PCR.jpg|500px]]
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Lane description
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|'''#'''
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|'''Description'''
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|'''Expected size (bp)'''
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|'''OK?'''
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|-
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|1
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|Smartladder
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|n/a
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|Yes
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|-
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|2-6
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|014
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|3000+
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|No
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|-
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|7-11
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|020
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|2100+
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|No
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|-
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|12-16
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|304
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|342
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|Maybe
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|-
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|17-21
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|327
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|310
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|Maybe
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|}

Revision as of 09:56, 27 August 2010

Strain Characterization

By Hugo

I'm working in some growth curves, today I tested a different amount of inoculum than yesterday.

Measurements!!!

Hour Time Glc Glc+CC6 C8
11:43 0h 0.015 0.014 0.007
14:54 3h 11' 0.042 0.052 0.066
16:00 4h 17' 0.099 0.081 0.086
17:00 5h 17' 0.196 0.128 0.142
18:00 6h 17' 0.355 0.215 0.184
19:00 7h 17' 0.555 0.341 0.275
9:43 22h 1.473 1.366 1.195
11:43 24h 1.459 1.360 1.398

Alkane Degradation parallel attempt

Yesterdays plates all contained colonies. But since this is a two way ligation, we expect a lot of religated backbones, so false positive colonies. That is why we performed a colony PCR.

We picked 5 colonies from each plate.

TU Delft 2010-08-18 Colony PCR.jpg

Lane description

# Description Expected size (bp) OK?
1 Smartladder n/a Yes
2-6 014 3000+ No
7-11 020 2100+ No
12-16 304 342 Maybe
17-21 327 310 Maybe
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