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Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology.
For more information look at the following websites:
www.nanovideohunt.nl
www.nanovideohunt.hyves.nl
www.nanopodium.nl
Lab work
By Hugo
I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform.
| #
| Code
| Resistance marker
| Function
|
| 1
| J61100
| Amp
| RBS Anderson family
|
| 2
| J61101
| Amp
| RBS Anderson family
|
| 3
| J61107
| Amp
| RBS Anderson family
|
| 4
| J61117
| Amp
| RBS Anderson family
|
| 5
| J61127
| Amp
| RBS Anderson family
|
| 6
| B0034
| Amp
| RBS
|
| 7
| J23109
| Amp
| Promoter Anderson family
|
| 8
| B0032
| Amp
| RBS Anderson family
|
| 9
| R0011
| Amp
| LacI promoter
|
| 10
| B0015
| Amp, Kan
| Double terminator
|
| 11
| R0010
| Amp
| LacI
|
| 12
| E0422
| Amp
| YFP generator
|
| 13
| E0240
| Amp
| GFP generator
|
| 14
| E0040
| Amp
| GFP
|
| 15
| I13401
| Amp
| GFP + Double terminator
|
Procedure:
- Add 1 ul of DNA to 33 ul of competent cells
- Put the tubes on ice for 10 min
- Warm the tubes in a water bath at 37º C for 5 min.
- Add 250 ul of LB medium
- Incubate the cells 1 hour at 37ºC, shake at 160 rpm
- Plate 200 ul over selective medium
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