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Team:TU Delft/16 July 2010 content
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Data analysis to follow shortly (good stuff!) | Data analysis to follow shortly (good stuff!) | ||
| - | <h5>Assembly of reference construct & | + | <h5>Assembly of reference construct & positive control</h5> |
| + | [https://2010.igem.org/Team:TU_Delft#/blog?blog=15_July_2010 Yesterday's] digestion products of K081005 and I13401 were set for ligation over the weekend. | ||
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The [[Team:TU_Delft/protocols/birnboim_plasmid_isolation|Birnboim method]] was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained: | The [[Team:TU_Delft/protocols/birnboim_plasmid_isolation|Birnboim method]] was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained: | ||
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Revision as of 22:48, 19 July 2010
Contents |
Lab work
Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing
The colony PCR of yesterday was put on1% agarose gel
We harvested the 1 mL bacterial cells of possible correct transformations. The pellets were stored at -20 °C. We used 3 mL of the bacterial cells to make -80 °C stocks.
Characterization of Anderson RBS sequences
Fluorescence measurements Attempt #2
Data analysis to follow shortly (good stuff!)
Assembly of reference construct & positive control
Yesterday's digestion products of K081005 and I13401 were set for ligation over the weekend.
The Birnboim method was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained:
| BioBrick | Concentration (ng/μL) |
| J23100 | |
| I13522 |
